![]() ![]() This method was applied successfully to construct a prokaryotic expression plasmid from four modules and a plant expression plasmid from five modules (fragments). This method is flexible to construct different types of plasmids because the modules can be freely selected and assembled in any combination. Imported File Types Lasergene Protein(.pro), Lasergen Multi-Seq Files (.mseq) MacVector Format (.nucl. After polymerase chain reaction (PCR) amplification for flanking with BsaI sites, multiple linear DNA components (modules) can be parallel assembled into a circle plasmid by a single restriction–ligation reaction using the method. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Unlike the original Golden Gate cloning system for cloning from entry vector to expression vector, this method can be used to construct plasmids immediately from linear DNA fragments. Flagging reads for assembly by flye in macvector Assembly Project. We present here a modified Golden Gate cloning method for modular construction of plasmids. Beautiful plasmid maps, simple primer design, drag and drop cloning and much. MACVECTOR ASSEMBLE PLASMID SOFTWARESequence analyses were performed by using the MacVector 8.0 software of the Oxford Molecular Group. Gap closure was performed by combinatorial PCR and sequencing of the generated PCR fragments. The common method for constructing plasmids is to cut DNA fragments by restriction enzymes and then join the resulting fragments using ligase. The data assembly was accomplished by using Staden Package software version 4.6 (Roger Staden, Cambridge, United Kingdom). Construction of plasmids is the basic and pivotal technology in molecular biology. ![]()
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